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active raf 1 ee  (R&D Systems)


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    Structured Review

    R&D Systems active raf 1 ee
    Active Raf 1 Ee, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/active+raf+1+ee/Recombinant+Human+Active+Raf-1+(Y340E+Y341E%2C+306-end)%2C+CF/pmc03404299-142-34-42
    Average 90 stars, based on 2 article reviews
    active raf 1 ee - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: BDNF Alters ERK/p38 MAPK Activity Ratios to Promote Differentiation in Growth Plate Chondrocytes
    Article Snippet: .. For the in vitro kinase assays, 10 ng of recombinant (His) 6 -tagged p38 protein (expressed in bacteria, a gift of Melanie H. Cobb) was incubated with either 10 ng of recombinant human constitutively active raf-1 EE (Y340E Y341E, 306-648) (purchased from R&D Systems, Minneapolis, MN) or with raf-1 immunoprecipitates from ATDC5 cell lysates as above, in 25 μl of kinase buffer plus 1 mg/ml BSA; after 30 min at 30 C, 0.5 μg of recombinant kinase-inactive GST-MEK and 5 μCi [γ- 32 P]ATP were added to these reactions, and the reactions were incubated for another 30 min at 30 C. Phosphate incorporation into MEK was assessed as above. .. For the in vitro inhibition of p38, 50 ng of (His) 6 -tagged p38 protein was incubated with or without 10 μ m SB203580 in the presence of 1:1000 anti-p38 antibody (Sigma-Aldrich, St. Louis, MO) and 20 μl of protein-A agarose; a parallel incubation was done with SB203580, antibody, and protein-A agarose but no p38 protein.

    Recombinant:

    Article Title: BDNF Alters ERK/p38 MAPK Activity Ratios to Promote Differentiation in Growth Plate Chondrocytes
    Article Snippet: .. For the in vitro kinase assays, 10 ng of recombinant (His) 6 -tagged p38 protein (expressed in bacteria, a gift of Melanie H. Cobb) was incubated with either 10 ng of recombinant human constitutively active raf-1 EE (Y340E Y341E, 306-648) (purchased from R&D Systems, Minneapolis, MN) or with raf-1 immunoprecipitates from ATDC5 cell lysates as above, in 25 μl of kinase buffer plus 1 mg/ml BSA; after 30 min at 30 C, 0.5 μg of recombinant kinase-inactive GST-MEK and 5 μCi [γ- 32 P]ATP were added to these reactions, and the reactions were incubated for another 30 min at 30 C. Phosphate incorporation into MEK was assessed as above. .. For the in vitro inhibition of p38, 50 ng of (His) 6 -tagged p38 protein was incubated with or without 10 μ m SB203580 in the presence of 1:1000 anti-p38 antibody (Sigma-Aldrich, St. Louis, MO) and 20 μl of protein-A agarose; a parallel incubation was done with SB203580, antibody, and protein-A agarose but no p38 protein.

    Bacteria:

    Article Title: BDNF Alters ERK/p38 MAPK Activity Ratios to Promote Differentiation in Growth Plate Chondrocytes
    Article Snippet: .. For the in vitro kinase assays, 10 ng of recombinant (His) 6 -tagged p38 protein (expressed in bacteria, a gift of Melanie H. Cobb) was incubated with either 10 ng of recombinant human constitutively active raf-1 EE (Y340E Y341E, 306-648) (purchased from R&D Systems, Minneapolis, MN) or with raf-1 immunoprecipitates from ATDC5 cell lysates as above, in 25 μl of kinase buffer plus 1 mg/ml BSA; after 30 min at 30 C, 0.5 μg of recombinant kinase-inactive GST-MEK and 5 μCi [γ- 32 P]ATP were added to these reactions, and the reactions were incubated for another 30 min at 30 C. Phosphate incorporation into MEK was assessed as above. .. For the in vitro inhibition of p38, 50 ng of (His) 6 -tagged p38 protein was incubated with or without 10 μ m SB203580 in the presence of 1:1000 anti-p38 antibody (Sigma-Aldrich, St. Louis, MO) and 20 μl of protein-A agarose; a parallel incubation was done with SB203580, antibody, and protein-A agarose but no p38 protein.

    Incubation:

    Article Title: BDNF Alters ERK/p38 MAPK Activity Ratios to Promote Differentiation in Growth Plate Chondrocytes
    Article Snippet: .. For the in vitro kinase assays, 10 ng of recombinant (His) 6 -tagged p38 protein (expressed in bacteria, a gift of Melanie H. Cobb) was incubated with either 10 ng of recombinant human constitutively active raf-1 EE (Y340E Y341E, 306-648) (purchased from R&D Systems, Minneapolis, MN) or with raf-1 immunoprecipitates from ATDC5 cell lysates as above, in 25 μl of kinase buffer plus 1 mg/ml BSA; after 30 min at 30 C, 0.5 μg of recombinant kinase-inactive GST-MEK and 5 μCi [γ- 32 P]ATP were added to these reactions, and the reactions were incubated for another 30 min at 30 C. Phosphate incorporation into MEK was assessed as above. .. For the in vitro inhibition of p38, 50 ng of (His) 6 -tagged p38 protein was incubated with or without 10 μ m SB203580 in the presence of 1:1000 anti-p38 antibody (Sigma-Aldrich, St. Louis, MO) and 20 μl of protein-A agarose; a parallel incubation was done with SB203580, antibody, and protein-A agarose but no p38 protein.



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    active raf 1 ee  (R&D Systems)
    90
    R&D Systems active raf 1 ee
    Active Raf 1 Ee, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/active+raf+1+ee/Recombinant+Human+Active+Raf-1+(Y340E+Y341E%2C+306-end)%2C+CF/pmc03404299-142-34-42
    Average 90 stars, based on 1 article reviews
    active raf 1 ee - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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